A simple, rapid, precise, accurate and reproducible high performance liquid chromatographic method was developed for estimation of flurbiprofen and chloramphenicol in eye drops. Chromatographic separation of the drugs was achieved on a C-18 phenomenex column using a mobile phase, methanol and 0.5% K2HPO4 (pH -8, adjusted with ortho phosphoric acid) in the ratio of 70:30 v/v, at a flow rate of 1.1 ml/min. The detection was carried out at 245nm. Chloramphenicol and Flurbiprofen were eluted at the retention time of 4.1 min and 6.6 min. The calibration curve of chloramphenicol and flurbiprofen were linear over the range of 10 µg – 800 μg/ml and 10 µg – 600 μg/ml respectively. The UV spectrophotometric (dual wavelength) method was performed at 213 nm and 262 nm for the determination of Flurbiprofen, 300 nm for the determination of chloramphenicol. The calibration curve of chloramphenicol and flurbiprofen were linear over the range of 10 µg – 800 μg/ml and 10 µg – 300 μg/ml respectively. Both methods were validated according to ICH guidelines. These methods can be applied for determination of titled drugs in its formulation with good accuracy and precision.
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